Epicypher——CUTANA™ CUT&RUN Library Prep Kit

8-strip Tubes

End Prep Enzyme

Ligation Enhancer

SPRIselectreagent manufactured by

Beckman Coulter, Inc.

End Prep Buffer

High Fidelity 2X PCR

Master Mix

0.1X TE Buffer

Adapter for Illumina®

U-Excision Enzyme

Ligation Mix

i5 and i7 Primers

FIGURE 1：

CUT&RUN DNA Fragment Size Distribution Analysis. CUT&RUN was  performed as described above. Library DNA was  analyzed by Agilent TapeStation® , which  confirmed that mononucleosomes were  predominantly enriched in CUT&RUN (~300 bp  peaks represent 150 bp nucleosomes +  sequencing adapters). Peaks at ~380 bp  correspond to the SNAP-CUTANA™ K-MetStat Panel of spike-in controls(EpiCypher 19-1002).

FIGURE 2：

Representative Gene Browser Tracks. CUT&RUN was performed as described above. A representative 674 kb window at the SEPTIN5 gene is shown for three replicates ("Rep") of IgG and H3K4me3 antibodies, as well as individual tracks for H3K27me3 and the transcription factor CTCF, demonstrating the robustness and reproducibility of the workflow with a variety of targets. Sequencing libraries prepared with the CUTANA CUT&RUN Library Prep kit produced the expected genomic distribution for each target. Images were generated using the Integrative Genomics Viewer (IGV, Broad Institute)

14-1001

CUTANA™ CUT&RUN Library Prep Kit with Primer Set 1

48 Reactions

14-1002

CUTANA™ CUT&RUN Library Prep Kit with Primer Set 2

48 Reactions