Vactorlabs：VectaPlex常見(jiàn)問(wèn)題解答

VectaPlex™ Antibody Removal Kit (VRK-1000)

Q: VectaPlex™可用于冰凍切片嗎?

A: No. During development, VectaPlex™ was optimized for FFPE tissues, where the power to remove antibodies at room temperature was balanced with the need to preserve tissue morphology. For frozen tissues, VectaPlex™ will remove the tissue from the slide.

Q: VectaPlex™ 可以去除免疫組化 (IHC) 實(shí)驗(yàn)應(yīng)用中的抗體染色（酶顯色）嗎？

A: VectaPlex™ Antibody Removal Kit is designed to be used in fluorescent workflows such as immunofluorescence or TSA. It has not been validated or confirmed for immunohistochemistry (IHC) applications. VectaPlex™ may remove substrates already deposited on tissue samples.

Q: 我有使用TrueView試劑盒去除組織的自發(fā)熒光, 請(qǐng)問(wèn)TrueVIEW會(huì)被VectaPlex™試劑去除嗎?

A: We used TrueView in our VectaPlex™ IF workflow to suppress autofluorescence. Application of VectaPlex™ removes some, but not all, of the TrueView reagent. Since some TrueView remained after VectaPlex™ application, we did not add TrueView in every cycle, but only as needed to suppress autofluorescence.

Q: 請(qǐng)問(wèn)將VectaPlex™試劑盒放置到4℃冰箱一段時(shí)間，還可以使用嗎?

A: Yes. VectaPlex™can withstand a freeze-thaw cycle and still perform as expected, but the recommended storage is at ambient temperature (15-25 °C). Additionally, the kit will still perform properly if placed at 4 °C.

Q: 在同一張切片上使用 VectaPlex™,是否有最大次數(shù)限制呢？

A: VectaPlex™ has been tested for up to 6 cycles with no ill effects. We don’t expect there to be issues with increasing the number of cycles, but we suggest testing with controls for adequate comparisons. We recommend running experiments to confirm that the use of more cycles does not impact the antigenicity or tissue morphology.

Q: VectaPlex™可用于自動(dòng)化染色平臺(tái)嗎?

A: We have not tested this product on automated systems and cannot guarantee instrument compatibility at this time.

Q: VectaPlex™ 是否適用于所有種屬來(lái)源的抗體?

A: VectaPlex™ has been tested with mouse and rabbit primaries, and with horse and goat secondaries. We expect it to work well with other species of antibodies as well. 

Q: VectaPlex™ 是否適用于不同類(lèi)型的組織樣本?

A: VectaPlex™ has been tested on and works well with various FFPE tissues, including FFPE breast and lung tissue which are very fragile.

Q: VectaPlex™ 是否與所有抗體相兼容?

A: VectaPlex™ has been tested on more than 30 common antibodies and has worked on them without issue.

Q: 在IF實(shí)驗(yàn)中, VectaPlex™ 抗體去除試劑是否也可以去除組織的自發(fā)熒光呢？

A: VectaPlex™ does not appear to reduce autofluorescence background.

Q: 是否可以提供使用VectaPlex™操作過(guò)程中移去蓋玻片的操作流程呢?

A: We recommend using VectaShield® Plus Antifade Mounting Media with DAPI (H-2000), which is an aqueous non-hardening mounting medium. After each imaging cycle, the coverslip can be easily removed by incubating in PBS and the residual VectaShield® removed with a PBS wash.