Sensitive measurement of Corticosterone, detecting as little as 27 pg/ml
High throughput format with results in 3 hours for up to 39 samples
Broad dynamic range
Species independent
Routinely used to monitor stress levels in zoo animals
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The Corticosterone EIA kit is a colorimetric competitive enzyme immunoassay kit with results in 3 hours. Absorbance is read at 405 nm. The Corticosterone EIA kit has low cross-reactivity with related steroids. The broad dynamic range makes this kit ideal for a wide variety of sample matrices from any species.
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Product Details
| Sensitivity: | 27.0 pg/ml (range 32 - 20,000 pg/ml) |
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| Assay Time: | 3 hours |
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| Applications: | ELISA, Colorimetric detection
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| Application Notes: | For the quantitative determination of Corticosterone in culture supernatants, feces, plasma, serum, and saliva from any species. Cited sample types include cell lysate, urine and whole blood. |
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| Wavelength: | 405 nm |
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| Species reactivity: | Species independent
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| Crossreactivity: | Corticosterone (100%), Deoxycorticosterone (21.3%), Desoxycorticosterone (21.0%), Progesterone (0.46%), Testosterone (0.31%), Tetrahydrocorticosterone (0.28%), Aldosterone (0.18%), Cortisol (0.046%) and <0.03%: Pregnenolone, Estradiol, Cortisone, 11-dehydrocorticosterone acetate |
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| Shipping: | Blue Ice Not Frozen |
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| Long Term Storage: | +4°C |
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| Contents: | DxS Microtiter plate, Conjugate, Antibody, Assay buffer 15 concentrate, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution, Steroid displacement reagent |
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| Scientific Background: | Corticosterone is a glucocorticoid secreted by the cortex of the adrenal gland. Corticosterone is produced in response to the stimulation of the adrenal cortex by adrenocorticotropic hormone (ACTH) and is the precursor of aldosterone. Corticosterone production has been shown to increase with stress, with elevated corticosterone levels being associated with impairment of long term memory retrieval and following burn injury. In addition to stress levels, corticosterone is believed to play a decisive role in sleep-wake patterns. |
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| Technical Info/Product Notes: | Cited samples:
Hormone ELISAs: Cited Samples
Wildlife Endocrinology: Cited Sample Types |
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| Protocol: | Suggested Small Volume Protocol for Serum/Plasma Samples
Let all solutions come to room temp before use. Aliquot 10µl of each sample into microfuge tube. Make 1ml 1:100 Steroid Displacement Reagent (SDR) solution (immediately before use) in deionized water or PBS (not assay buffer) Add 10µl 1:100 SDR to each sample tube. Vortex, let stand >5 minutes before diluting with EIA buffer. Add 380µl EIA assay buffer to each plasma tube, vortex.
Final dilution is ~1:40 (This final dilution may not be appropriate for every sample; thus, optimal dilution must be determined by each end user for their experimental sample group.) |
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| Regulatory Status: | RUO - Research Use Only |
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| Compatibility: | This product is compatible with the Absorbance 96 Plate Reader. |
Product Literature References
A comparison of white/red and white/blue LED light fixtures on Pekin duck production, stress and behaviour: G.M. House, et al.; Br. Poult. Sci. (2021), Abstract;
Adolescent Intermittent Ethanol (AIE) Produces Sex Specific Alterations in Adult Neuroimmune Gene Expression and Ethanol Sensitivity that are independent of Ethanol Metabolism: A.S. Vore, et al.; Neuropharmacology 195, 108635 (2021), Abstract;
Are individuals consistent? Endocrine reaction norms under different ecological challenges: D. Baldan, et al.; J. Exp. Biol. (2021), Abstract;
Breastfeeding undernutrition changes iBAT-involved thermogenesis protein expression and leads to a lean phenotype in adult rat offspring: G.D.S. Miranda, et al.; J. Nutr. Biochem. 99, 108857 (2021), Abstract;
Callous unemotional trait-like mice and their stressed dams: G. Laviola, et al.; Psychoendocrinol. 131, 105296 (2021), Abstract;
Chronic social defeat stress causes retinal vascular dysfunction: M. Wang, et al.; Exp. Eye Res. 213, 108853 (2021), Abstract;
Chronic social defeat stress causes retinal vascular dysfunction: M. Wang, et al.; Exp. Eye Res. 213, 108853 (2021), Abstract;
Developmental conditions have intergenerational effects on corticosterone levels in a passerine: F. H. Kraft, et al.; Horm. Behav. 134, 105023 (2021), Abstract;
Dietary Milk Phospholipids Attenuate Chronic Stress-Induced Changes in Behavior and Endocrine Responses across the Lifespan: M. Schverer, et al.; Mol. Nutr. Food Res. (2021), Abstract;
Dietary Milk Phospholipids Attenuate Chronic Stress-Induced Changes in Behavior and Endocrine Responses across the Lifespan: M. Schverer, et al.; Mol. Nutr. Food Res. (2021), Abstract;
Differential effects of noise exposure between substrains of CBA mice: C.P.C. Versteegh, et al.; Hear Res. (2021), Abstract;
Differential effects of noise exposure between substrains of CBA mice: C.P.C. Versteegh, et al.; Hear Res. (2021), Abstract;
Effect of extremely low frequency magnetic fields on oxidative balance in rat brains subjected to an experimental model of chronic unpredictable mild stress: L.R. Quesnel-Galván, et al.; BMC Neurosci. 22, 52 (2021), Abstract;
Effects of Scopoletin Supplementation and Stocking Density on Growth Performance, Antioxidant Activity, and Meat Quality of Korean Native Broiler Chickens: S. H. Ha, et al.; Foods 10, 1505 (2021), Abstract;
Evaluation of Alternative Euthanasia Methods of Neonatal Chickens: G. Shailesh, et al.; Animals 8, 37 (2021), Abstract; Full Text
Experimental monochromatic light-emitting diode fixture impacts Pekin duck stress and eye development: G.M. House, et al.; Poultry Sci. 100, 101507 (2021), Abstract;
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