G418 (solution)

G418 (Geneticin) : selection antibiotic, endotoxin tested, sterile reagent

G418 also known as G-418, G418 sulfate or Geneticin is an aminoglycoside antibiotic produced by Micromonospora rhodorangea.

G418 blocks polypeptide synthesis by inhibiting the elongation step in both prokaryotic and eukaryotic cells.

Resistance to G418 is conferred by the Neomycin resistance gene (neo) from Tn5 encoding an aminoglycoside 3'-phosphotransferase, APH 3' II.

Selection in mammalian cells is usually achieved in 3 to 7 days with concentrations ranging from 400 to 1000 µg/ml.

Cells that are dividing are affected more rapidly than non-dividing cells.

Details

WORKING CONCENTRATIONS

G418 is normally used at a concentration of 400 μg/ml. However, the optimal concentration needs to be determined for your cells.
Suggested concentrations of G418 for selection in some examples of mammalian cells are listed below:

References:

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